The refuge has three easy trails that provide wildlife viewing opportunities, passing through cypress groves, wetlands, and pine forests.
The New Holland Trail passes through cypress groves and wetlands and is the ideal place for connecting with nature. The Outfall Trail provides excellent views of the historic Mattamuskeet Lodge and passes through pine woods. The Photo Blind Trail is lined with tall grasses on the path to a blind for photography located on the bank of Lake Mattamuskeet.
The Outfall Trail is a grass-surface trail, 0.25mi round trip trail .ocated just inside the gate off New Holland/Mattamuskeet Road. The trail begins at the bridge by the New Holland Pumphouse/Mattamuskeet Lodge historic building parking area and picnic grounds. Traverse along the pond created by the outfall canal, where it is easy to watch great egret and great blue heron. The trail is not marked but there are two peaceful habitat viewing benches.