U.S. Fish & Wildlife Service Guidance Documents

This is a database of U.S. Fish and Wildlife Service guidance documents that are statements of general applicability, intended to have future effect on the behavior of regulated parties, that set forth policy on a statutory, regulatory, or technical issue, or an interpretation of a statute or regulation.

If you have a question or suggestion about these guidance documents, please send an email to guidance_webportal@fws.gov. To ensure adequate response, please include the title of the document and its ID number in your email.


Details and documents Program Date issued Date posted

17α-Methyltestosterone Rainbow Trout and Atlantic Salmon Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for the use of 17-alpha methyltestosterone in rainbow trout and Atlantic salmon (INAD #8557). It is a revision to a protocol published 7/16/2007.

ID: FW-G-0058

Fisheries and Aquatic Conservation

17α-Methyltestosterone Tilapia Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for the use of 17-alpha methyltestosterone in tilapia (INAD #11-236). This is a revision to an existing protocol published in 2007.

ID: FW-G-0059

Fisheries and Aquatic Conservation

17β-Estradiol Brook Trout Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for the use of 17-beta estradiol in brook trout (INAD #12-671). This is a revised protocol that was published in 10/2016.

ID: FW-G-0060

Fisheries and Aquatic Conservation

35% Perox-Aid (Hydrogen Peroxide) Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for the use of 35% Perox-Aid (hydrogen peroxide) for the control of ectoparasites (INAD #11-669). This is a revised protocol first published in 12/2007.

ID: FW-G-0061

Fisheries and Aquatic Conservation

Administering Concessions (630 FW 8)

Agency policy for administering concessions, including day-to-day operations and oversight.

ID: FW-G-0001

National Wildlife Refuge System

Administration of National Wildlife Refuge System Conservation Easements (601 FW 6)

National policy for administration of conservation easements.

ID: FW-G-0002

National Wildlife Refuge System

Administrative Actions to Strengthen U.S. Trade Controls for Elephant Ivory, Rhinoceros Horn, and Parts and Products of Other Species Listed Under the Endangered Species Act, as amended (Director's Order 210)

This document describes administrative Actions to Strengthen U.S. Trade Controls for Elephant Ivory, Rhinoceros Horn, and Parts and Products of Other Species Listed Under the Endangered Species Act (ESA).

ID: FW-G-0079

International Affairs

Appropriate Refuge Uses (603 FW 1)

National framework for determining appropriate refuge uses.

ID: FW-G-0004

National Wildlife Refuge System

Aquaflor as a Feed Additive Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for Aquaflor (forfenicol) use as a feed additive (INAD #10-697). This is a revision to a protocol published in 2/2009.

ID: FW-G-0062

Fisheries and Aquatic Conservation

Aqui-S 20E Investigational New Animal Drug (INAD) Study Protocol

This is a revision to an existing Investigational New Animal Drug (INAD) study protocol published in 2013 and revised in 2016 and 2021 for the use of Aqui-S 20E (eugenol) (INAD #11-741).

ID: FW-G-0063

Fisheries and Aquatic Conservation

Assistance Awards Guidelines - Division of International Conservation

Guidelines for recipients of financial assistance awards issued by the U.S. Fish and Wildlife Service’s (Service) International Affairs programs. 

ID: FW-G-0080

International Affairs

Benzoak Vet (Benzocaine) Exemption Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for a compassionate aquaculture exemption for Benzoak Vet (benzocaine).

ID: FW-G-0232

Fisheries and Aquatic Conservation

Biological Integrity, Diversity, and Environmental Health, as amended (601 FW 3)

Policy for maintaining and restoring biological integrity, diversity, and environment health on national wildlife refuges.

ID: FW-G-0005

National Wildlife Refuge System

Calcein (Se-Mark) Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for the use of Calcein (Se-Mark) (INAD #10-987).

ID: FW-G-0064

Fisheries and Aquatic Conservation

Chloramine-T Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for the use of Chloramine-T (INAD #9321). This is a revision to a protocol published in September 2007.

ID: FW-G-0066

Fisheries and Aquatic Conservation

Coastal Barrier Resource System (651 FW 1)

Guidance for administering the Coastal Barrier Resources Act.

ID: FW-G-0007

National Wildlife Refuge System

Coastal Zone Management (613 FW 3)

Policy and guidance on compliance with the Coastal Zone Management Act of 1972

ID: FW-G-0008

National Wildlife Refuge System

Common Carp Pituitary (CCP) Investigational New Animal Drug (INAD) Study Protocol

This is a revision to an existing protocol published in 2020 and revised in 8/2021. It is an Investigational New Animal Drug (INAD) study protocol for the use of Common Carp Pituitary (CCP) (INAD #8391). 

ID: FW-G-0067

Fisheries and Aquatic Conservation

Compatibility Determination for Refuge Uses (603 FW 2)

Policy to determine compatibility of proposed and existing uses of national wildlife refuges.

ID: FW-G-0009

National Wildlife Refuge System

Compliance with National Historic Preservation Act (614 FW 3)

Guidance on compliance with Section 106 of the National Historic Preservation Act on national wildlife refuges.

ID: FW-G-0010

National Wildlife Refuge System

Comprehensive Conservation Planning Process (602 FW 3)

Guidance on developing a Comprehensive Conservation Plan (CCP) for refuges.

ID: FW-G-0011

National Wildlife Refuge System

Cooperative Agricultural Use (620 FW 2)

Policy for use of cooperative agriculture on national wildlife refuges.

ID: FW-G-0012

National Wildlife Refuge System

Cooperative Agriculture Program Guidance for Region

This guidance assists staff in developing and implementing a cooperative agriculture program on their refuges.

ID: FW-G-0006

National Wildlife Refuge System

Diquat Dibromide Investigational New Animal Drug (INAD) Study Protocol

This is a second revision of an existing Investigational New Animal Drug (INAD) study protocol that was published in 2007. It is for the use of Reward (diquat dibromide) (INAD #10-969).

ID: FW-G-0068

Fisheries and Aquatic Conservation

E-Permits Online Submission

Instructions on how to submit an application form are available by clicking on "help" including Frequently Asked Questions and a Quick Start Guide

ID: FW-G-0081

International Affairs

Eagle Conservation Plan Guidance, Module 1-Land-based Wind Energy

This document provides guidance for wind developers seeking permits for incidental take of bald and golden eagles.

ID: FW-G-0088

Migratory Bird Program

Eligibility of State Grant Projects for the Stocking of Pen-Raised Birds Under the Pittman-Robertson Wildlife Restoration Act

Interprets the Wildlife Restoration Act for eligibility for projects for stocking pen-raised birds, and also gives requirements for projects.

ID: FW-G-0096

Wildlife and Sportfish Restoration Program

Endangered Species Act Compensatory Mitigation Policy

This policy provides clarity and predictability to Service staff and project proponents on applying compensatory mitigation in the context of the ESA to offset effects to federally listed, proposed, or at risk-species, and proposed or designated critical habitat. It also should assist other Federal agencies in carrying out their statutory and regulatory responsibilities under the ESA and should provide project proponents with guidance on the appropriate use of compensatory mitigation for proposed actions.

ID: FW-G-0233

Ecological Services (Endangered Species)

Erythromycin 200 Injectable Investigational New Animal Drug (INAD) Study Protocol

This an additional revision to a study protocol published in 2016 and revised in August of 2021. It is an Investigational New Animal Drug (INAD) study protocol for the use of Erythromycin (Erymicin 200 injection) (INAD #12-781).

ID: FW-G-0069

Fisheries and Aquatic Conservation

Establishment, Use, and Operation of Conservation Banks Guidance

This guidance intends to help Service personnel evaluate proposals to establish conservation banks.

ID: FW-G-0048

Ecological Services (Endangered Species)

Federal Land Managers Air Quality Related Values Working Group Phase 1 Report

This report, housed on the National Park Service's site, was co-developed to provide a consistent approach to air quality modeling and analysis among Federal land managers.

ID: FW-G-0013

National Wildlife Refuge System

Field Trials for Hunting (631 FW 5)

Policy for permitting field trials for recreational hunting on national wildlife refuges.

ID: FW-G-0014

National Wildlife Refuge System

Finance and Administration through Friends Agreements (633 FW 2)

Agency policy and procedures related to finance and administrating work with Friends organizations.

ID: FW-G-0015

National Wildlife Refuge System

Floodplain Management (613 FW 1)

Policy and guidance on compliance with Executive Order 11988, Floodplain Management.

ID: FW-G-0016

National Wildlife Refuge System

Friends Nature Stores and Other Revenue Generating Activity (633 FW 4)

Agency policy on how to interact with Friends organizations when they run nature stores and other revenue generating initiatives.

ID: FW-G-0017

National Wildlife Refuge System

Friends Partnership Agreements (633 FW 3)

Agency policy on how to use Friends agreements and what they include.

ID: FW-G-0018

National Wildlife Refuge System

General Instructions for Registering in DUNS / System of Award Management (SAM)

Instructions for recipients on how to create a DUNS number for their organization, and register in SAM. 

ID: FW-G-0082

International Affairs

GnRH IIa Chicken Gonadotropin-Releasing Hormone Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for GnRH IIa chicken gonadotropin-releasing hormone II analog use (INAD #13-345).

ID: FW-G-0230

Fisheries and Aquatic Conservation

Grant Agreement Requirements and Program Income Guidance for Third-Party Commitments Under the State Wildlife Grants Program and the Landowner Incentive Program

Tells State Wildlife Grant and Landowner Incentive Program recipients how they must document and maintain a record of third-party agreements and determine and treat program income.

ID: FW-G-0097

Wildlife and Sportfish Restoration Program

Guidance for Wildlife Action Plan Review and Revisions

Tells States the process for updating State Wildlife Action Plans in order to receive benefits under the State Wildlife Grant Program.

ID: FW-G-0098

Wildlife and Sportfish Restoration Program

Guidance on the Antique Exception Under the Endangered Species Act, as amended (Appendix 1 to Director's Order 210)

Guidance on the Antique Exception Under the Endangered Species Act for certain protected species (ivory).

ID: FW-G-0083

International Affairs

Habitat and Species Step-Down Planning (602 FW 6)

Policy for planning and developing habitat management plans and annual habitat work plans on national wildlife refuges.

ID: FW-G-0019

National Wildlife Refuge System

Habitat Conservation Planning and Incidental Take Permitting Processing Handbook (Joint Release/FWS and NMFS)

The purpose of this handbook is to serve as an instructional aid for Fish and Wildlife Service and National Marine Fisheries Service staff. It should be equally helpful to other Habitat Conservation Plan practitioners, such as applicants, consultants, and partners.

ID: FW-G-0051

Ecological Services (Endangered Species)

Implementation of the D.C. Circuit Court Decision in United States Association of Reptile Keepers, Inc. v. Zinke, No. 15-5199 (D.C. Cir. )

This document explains to the public how the Department of the Interior implements the court decision for injurious wildlife. It is related to the interpretation of interstate transport in 18 U.S.C. 42 (a). 

ID: FW-G-0070

Fisheries and Aquatic Conservation

Incidental Take of Migratory Birds (Director's Order 225)

This policy, which goes into effect on 12/3/2021, explains that the Service interprets the Migratory Bird Treaty Act (MBTA) to prohibit incidental take of migratory birds and will enforce the statute accordingly. This Order provides background and guidance to Service employees, including expectations for conducting Service activities, providing technical assistance, and prioritization of our enforcement activities.

ID: FW-G-0231

Migratory Bird Program

Interim Guidance - Implementing the Target Practice and Marksmanship Training Act (Public Law 116-17)

Gives guidance to States on how to implement the amendments to the Wildlife Restoration Act as a result of Public Law 116-17. Guidance will be rescinded when a final rule is published.

ID: FW-G-00100

Wildlife and Sportfish Restoration Program

Interim Guidance: Implementing the Modernizing the Pittman-Robertson Fund for Tomorrow's Needs Act

Gives guidance to States on how to implement the amendments to the Wildlife Restoration Act as a result of Public Law 116-94. Will be rescinded when a final rule is published.

ID: FW-G-0099

Wildlife and Sportfish Restoration Program

Inventory and Monitoring in the National Wildlife Refuge System (701 FW 2)

Guidance on how natural resource surveys are conducted, including collaboration with survey partners.

ID: FW-G-0020

National Wildlife Refuge System

Luteinizing Hormone-Releasing Hormone (LHRHa) Investigational New Animal Drug (INAD) Study Protocol

This is a second revision to an existing study protocol published 3/2021 and revised 9/2021. It is an Investigational New Animal Drug (INAD) study protocol for the use of Luteinizing Hormone-Releasing Hormone analog (LHRHa) (INAD #8061). 

ID: FW-G-0071

Fisheries and Aquatic Conservation

Management of Environmental Education Programs (605 FW 6)

Policy on management of environmental education programs on national wildlife refuges.

ID: FW-G-0021

National Wildlife Refuge System

Management of Interpretive Programs (605 FW 7)

Policy on management of interpretive programs on national wildlife refuges.

ID: FW-G-0022

National Wildlife Refuge System

Management of Recreational Fishing Programs (605 FW 3)

Policy on management of recreational fishing programs on national wildlife refuges.

ID: FW-G-0023

National Wildlife Refuge System

Management of Recreational Hunting Programs (605 FW 2)

Policy on management of recreational hunting programs on national wildlife refuges.

ID: FW-G-0024

National Wildlife Refuge System

Management of Recreational Wildlife Photography Programs (605 FW 5)

Policy on management of recreational wildlife photography programs on national wildlife refuges.

ID: FW-G-0025

National Wildlife Refuge System

Management of Wildlife Observation Programs (605 FW 4)

Policy on management of wildlife observation programs on national wildlife refuges.

ID: FW-G-0026

National Wildlife Refuge System

Managing Cultural Resources Overview (614 FW 1)

Policy for managing cultural resources on national wildlife refuges.

ID: FW-G-0027

National Wildlife Refuge System

Measures of Effectiveness for Wildlife Conservation in Central America - Latin America Regional Program (English and Spanish)

The document identifies indicators for both conservation actions and threats. It seeks to serve as draft guidelines for how to measure the effectiveness of commonly-funded conservation actions and how to assess the status of common threats across the region. 

ID: FW-G-0084

International Affairs

Minerals Management (612 FW 1)

Policy for managing development of minerals that exist beneath national wildlife refuges.

ID: FW-G-0028

National Wildlife Refuge System

Mitigation Policy

This policy is intended to protect and conserve the most important and valuable fish and wildlife resources while facilitating balanced development of the Nation's natural resources.

ID: FW-G-0052

Ecological Services (Endangered Species)

National Bald Eagle Management Guidelines

Document provides guidance for avoiding impacts to bald eagles that could result in take in the form of distubance.

72 FR 31156 

 

ID: FW-G-0089

Migratory Bird Program

Native American Policy (510 FW 1)

The Native American Policy provides a framework for government-to-government relationships, which furthers the United States’ and the Department of the Interior’s trust responsibility to federally recognized tribes to protect, conserve, and use tribal reserved, treaty guaranteed, or statutorily identified resources.

ID: FW-G-0095

Native American Program

Ovaplant Salmon Gonadotropin-Releasing Hormone Analogue (sGnRHa) Investigational New Animal Drug (INAD) Study Protocol (INAD #11-375)

This is an Investigational New Animal Drug (INAD) study protocol for the use of Salmon Gonadotropin-Releasing Hormone analog (sGnRHa-Ovaplant) (INAD #11-375).

ID: FW-G-0072

Fisheries and Aquatic Conservation

Ovaplant-L Salmon Gonadotropin-Releasing Hormone Analogue (sGnRHa) Investigational New Animal Drug (INAD) Study Protocol (INAD #13-298)

This is an Investigational New Animal Drug (INAD) study protocol for the use of Salmon Gonadotropin-Releasing Hormone analog (sGnRHa-Ovaplant-L) (INAD #13-298). This is a revision to the protocol published 6/2019.

ID: FW-G-0073

Fisheries and Aquatic Conservation

Overview of Concessions, Authorities, and Responsibilities (630 FW 6)

Agency policy for concession operations on national wildlife refuges. Overview includes information on authorities and responsibilities.

ID: FW-G-0029

National Wildlife Refuge System

Oxytetracycline Dihydrate Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for Oxytetracycline dihydrate (Terramycin 200) medicated feed (INAD #9332). It is a revision to a protocol published in April 2008.

ID: FW-G-0075

Fisheries and Aquatic Conservation

Oxytetracycline Hydrochloride Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for the use of Oxytetracycline (Terramycin 343) for immersion therapy (INAD #9033). This is a revision to the protocol published 7/2007.

ID: FW-G-0077

Fisheries and Aquatic Conservation

Partners for Fish and Wildlife Program (640 FW 1)

Policies and procedures for implementation of the Partners for Fish and Wildlife Program.

ID: FW-G-0030

National Wildlife Refuge System

Policy on Management of Permitted Cabins in Alaska

Alaska regional policy on permitted cabins.

ID: FW-G-0032

National Wildlife Refuge System

Policy, Procedures, and Responsibilities for Volunteers (150 FW 1)

Agency overall policy on working with volunteers.

ID: FW-G-0033

National Wildlife Refuge System

Real Property: Valuation, Negotiation, and Acquisition (520 FW 7)

Sets policies for the acquisition of real property in financially assisted projects administered by the Wildlife and Sport Fish Restoration Program. Guidance will be rescinded when superseded by regulations.

ID: FW-G-0101

Wildlife and Sportfish Restoration Program

Recovering Equipment Costs on a Wildlife and Sport Fish Restoration Grant

Informs recipients of requirements that must be met in order to recover costs for equipment used as part of a Wildlife and Sport Fish Restoration Program Federal award.

ID: FW-G-0102

Wildlife and Sportfish Restoration Program

Revised Special Use Permit Fees for Commercial Guiding on NWRs in Alaska

Guidance on revised Special Use Permit fees for commercial guiding, outfitting, and transporting operations on national wildlife refuges in Alaska.

ID: FW-G-0034

National Wildlife Refuge System

Slice Investigational New Animal Drug (INAD) Study Protocol

This is an Investigational New Animal Drug (INAD) study protocol for Slice (emamectin benzoate) use (INAD #11-370). It is a revision to a protocol published 5/2010.

ID: FW-G-0078

Fisheries and Aquatic Conservation

Soliciting and Awarding Concession Contracts (630 FW 7)

Agency policy for awarding concession contracts.

ID: FW-G-0035

National Wildlife Refuge System

Standard Measures of Effectiveness and Threats for Wildlife Conservation in Central Africa

Standard indicators were developed to improve conservation by helping USFWS and its grantees and partners to: monitor, assess and report on performance; collect, share and aggregate comparable data; and learn and improve from others implementing similar actions.

ID: FW-G-0085

International Affairs

U.S. Fish and Wildlife Service Land-Based Wind Energy Guidelines

These voluntary guidelines provide a structured, scientific process for addressing wildlife conservation concerns at all stages of land-based wind energy development.

ID: FW-G-0056

Ecological Services (Endangered Species)

Use of Non-Lead Ammunition for Management and Research Activities on Refuges (601 FW 8)

National policy for the use of non-lead ammunition for wildlife management and collections activities on national wildlife refuges. The policy does not apply to public hunting on refuges.

ID: FW-G-0036

National Wildlife Refuge System

Using Available Legal and Regulatory Authority to Deny Wildlife Violators from Obtaining Federal Wildlife-Related Permits and Licenses (Director's Order 212)

This Order establishes policy and procedure for U.S. Fish and Wildlife Service employees to assert our full legal and regulatory authority to deny wildlife violators the ability to obtain wildlife permits, certificates, and licenses.

ID: FW-G-0086

International Affairs

Volunteer Eligibility (150 FW 2)

Agency policy on requirements and documents necessary for working with volunteers.

ID: FW-G-0037

National Wildlife Refuge System

Volunteer Program Operations (150 FW 3)

Agency policy on day-to-day operations when volunteers are involved in activities.

ID: FW-G-0038

National Wildlife Refuge System

Wetlands Policy and Action Plan (660 FW 1)

Guidance on wetland policies and action plans.

ID: FW-G-0039

National Wildlife Refuge System

Wetlands Protection (613 FW 2)

Policy and guidance on compliance with Executive Order 11990, Protection of Wetlands.

ID: FW-G-0040

National Wildlife Refuge System

What Can I Do With My Ivory?

The information on this webpage is intended to provide guidance for those who wish to buy, sell, or otherwise trade in elephant ivory.

ID: FW-G-0087

International Affairs

Wilderness Administration and Resource Stewardship (610 FW 2)

Policy on wilderness administration and resource stewardship.

ID: FW-G-0041

National Wildlife Refuge System

Wildlife and Sport Fish Restoration Program Guidance on Third-Party Contracts for Personnel Services

Instructs grant recipients what they must do to support using donated personnel services under a contract as matching funds to the Federal award. 

ID: FW-G-0103

Wildlife and Sportfish Restoration Program

Wildlife-Dependent Recreation Guidelines (605 FW 1)

Overall policy on management of wildlife-dependent recreation programs on refuges.

ID: FW-G-0042

National Wildlife Refuge System

Working with Friends Organizations (633 FW 1)

Agency policy for establishing and working with Friends organizations, which are nonprofit organizations that specifically support a Service site or program.

ID: FW-G-0043

National Wildlife Refuge System