Plan Your Visit


The 6,446-acre Trempealeau National Wildlife Refuge lies within the Mississippi flyway, along the Mississippi River in western Wisconsin. It is an isolated backwater, cut off from the Mississippi and Trempealeau rivers by dikes. It is a resting and feeding area for waterfowl and other birds.

What To Do

Plan to explore the 4.5-mile self-guided Prairie’s Edge Tour Loop which is accessible by bikes, pedestrians and vehicles. Along the tour, visit the observation deck and look through the high-powered spotting scopes. There are several short self-guided walking trails and walking on top of the refuge dikes provide some excellent wildlife viewing opportunities. Plan to spend time browsing the book store in the Visitor Contact Station operated by the Friends of Trempealeau Refuge. Whether it is by foot, bicycle or car, let the refuge satisfy and soothe your spirit. 

Hours of Operation

Visitor Contact Station/Refuge Office Hours
7:30 am - 4:00 pm
Monday - Friday
Limited staffing may necessitate closing the office to accommodate duties out on the Refuge.

Trails and Grounds Hours
Dawn to Dusk


Contacting the Refuge
V/TTY: 800-877-8339

Getting Here

W28488 Refuge Road
Trempealeau, WI 54661

From La Crosse, Wisconsin, take Route 53 north to Route 54 west. Follow Route 54 to Centerville and continue 3.1 miles to West Prairie Road. Turn left on West Prairie Road and drive one mile to the refuge entrance. 

From Winona, Minnesota take Route 43 across the Mississippi River into Wisconsin. Turn right onto Route 54 east. Travel 5.7 miles to West Prairie Road. Turn right onto West Prairie Road and drive one mile to the refuge entrance.  

Know Before You Go

 Remember to bring:

  • Sunglasses
  • Bug Spray/Head Net
  • Hat
  • Water Bottle
  • Sunscreen
  • Comfortable Shoes for Walking/Hiking


Only service dogs are allowed in the visitor contact station. Dogs are welcome, on-leash, on Refuge grounds and trails. Pet owners are encouraged to pack-out excrement. 

Don't forget to checkout our Rules and Regulations

Federal Recreational Lands Passes

We no longer carry any of the federal recreational lands passes. Nearby recreation sites to obtain passes includes Upper Mississippi River National Wildlife and Fish Refuge in La Crosse, WI and Necedah National Wildlife Refuge. There is an error on the website stating we have these passes available. We do not have them available and have notified their website. For information about passes and where to obtain them click here. 

Neighboring Refuges