Fish and Aquatic Conservation

Slice® (Emamectin Benzoate) INAD #11-370

photo of Atlantic sturgeon swimming
Atlantic sturgeon at the Welaka National Fish Hatchery Credit: Ryan Hagerty/USFWS


SLICE® is an aquaculture premix containing emamectin benzoate and is only available through Merck Animal Health. SLICE® premix can be purchased through Merck Animal Health and sent to an aquaculture feed mill for top coating. The primary goal of field studies conducted under INAD #11-370 is to evaluate the efficacy of SLICE®-medicated feed and safety of SLICE® to control mortality caused by external parasites in a variety of freshwater and marine fish species. The treatment option that is allowed to administer SLICE® in feed is 50 µg EB per kg of fish biomass per day for 7 consecutive days, and the investigational withdrawal period for treated fish is 60 days. Note: SLICE doesn’t usually reach fully efficacy until 25-35 days after treatment depending on water temperature. This INAD requires all participants to be approved by the FDA Environmental Team prior to participation – this review will take 90 days. To participate in the INAD Program for SLICE® visit the data collecting site.

Please read the Study Protocol prior to any treatments. It contains the protocol, MSDS, and a copy of the forms to be used as a guide for collecting data that will be entered into the online INAD database. The FDA authorization letter(s) for this INAD is currently only available by emailing Bonnie Johnson.

INAD objective/purpose: Collect scientific data necessary to establish the effectiveness AND safety of SLICE® to control mortality caused by external parasites in a variety of freshwater fish species.

Drug Name
SLICE® (emamectin benzoate; 0.2% aquaculture premix)

Drug Source
Merck Animal Health
35500 W. 91st Street
De Soto, KS 66018

Kasha M. Cox
Phone: 662-907-0692

Target pathogen(s): parasitic copepods

Method of administration: Medicated feed treatment

Treatment dosage:

  • 50 micrograms (μg) emamectin benzoate per kilogram (kg) of fish biomass per day

Treatment regimen:

  • 7 consecutive days
  • SLICE® should be administered as a single treatment event, with no repetition of treatment.

Investigational Withdrawal period:

  • 60 days
  • No investigational withdrawal period is required for fish that will not be catchable for 60 or more days after release or are illegal for harvest during that 60 day period.

Required test parameters:

  • Investigator must collect mortality data throughout the 5 day pre-treatment, treatment, and 28 day post-treatment periods. Note: SLICE doesn't usually reach fully efficacy until 20-30 days after treatment depending on water temp. The case history and analyses of parasite load needs to be checked both pre- and post-treatment. Investigator must also report general fish behavior and any adverse effects relating to treatment.

Limitations or restrictions on use of drug:

  • Investigator must follow all instructions in the Study Protocol for INAD 11-370 regarding drug acquisition and handling, fish treatment and disposition, and data reporting requirements.
  • Investigator must complete Facility Effluent Information Form and obtain NPDES acknowledgement/permission for discharge of SLICE® before use under the INAD. Contact AADAP for more information.

Required INAD fee: $700/facility/year

AADAP Contact Information:
Ms. Bonnie Johnson, FWS-AADAP
Phone: 406-994-9905
Fax: 406-582-0242