Fish and Aquatic Conservation


GnRH IIa Chicken Gonadotropin – Releasing Hormone II analog INAD #13-345

photo of a Channel catfish
Adult Channel catfish. Credit: Eric Engbretson/USFWS

Summary

GnRH IIa is a synthetic peptide analogue of chicken gonadotropin-releasing hormone (cGnRH IIa). It is presented as a dry powder to be resuspended in saline solution for intraperitoneal (IP) injection and is only available for purchase through AquaTactics Fish Health. The use of hormones to induce spawning in fish is critical to the success of many aquatic programs that need hormone treatment to complete final gamete maturation to ensure spawning. The primary goal of field studies conducted under INAD #13-345 is to generate data to help determine appropriate GnRH IIa treatment regimens for use as a spawning aid for female ictalurids. Fish may be treated at a dose up to 100 microgram (µg) GnRH IIa per kilogram (kg) body weight with single or multiple IP injections. Multiple injections will generally consist of a single “priming dose,” followed by a single “resolving dose.” Adherence to a 1-day Investigational withdrawal period is required for all fish treated with GnRH IIa under this INAD. Note: if MS222 or Aqui-S 20E is used at time of injection, then the withdrawal time for the anesthetic must be followed. To participate in the INAD Program for GnRH IIa visit the data collecting site.

Please read the Study Protocol prior to any treatments. It contains the protocol, MSDS, and a copy of the forms to be used as a guide for collecting data that will be entered into the online INAD database. The FDA authorization letter(s) for this INAD is currently only available by emailing Bonnie Johnson.


INAD objective/purpose: Collect supportive and pivotal data needed to establish the effectiveness of GnRH IIa for use as a spawning aid for female ictalurids.

Drug Name
GnRH IIa; Chicken Gonadotropin - Releasing Hormone II analogue (cGnRHa)

Drug Source
DelTaq Fish Health LLC
PO Box 343
Stoneville, MS 38776
Email: tomg@deltaqfishhealth.com

Tom Goodrich/Rebecca Goyt
Phone: 425-629-8099
Fax: 425-629-8095
email: tomg@deltaqfishhealth.com or rebeccag@aquatactics.com
Website: www.deltaqfishhealth.com

Target pathogen(s): Not Applicable

Method of administration: IP injection

Treatment dosage:

  • Up to 100 microgram (μg) GnRH IIa per kilogram (kg) body weight.

Treatment regimen:

  • Single treatment

Investigational Withdrawal period:

  • 1-day investigational withdrawal period
  • Note: if MS222 or Aqui-S 20E is used at time of injection, then the withdrawal time for the anesthetic must be followed.

Required test parameters:

  • Investigator must collect data reporting percent ovulation in treated fish. Investigator should also report general fish behavior and any adverse effects relating to treatment.

Limitations or restrictions on use of drug:

  • Investigator must follow all instructions in the Study Protocol for INAD 13-345 regarding drug acquisition and handling, fish treatment and disposition, and data reporting requirements.
  • Drug discharge must be in compliance with local NPDES permitting requirements.

Required INAD fee: $700/facility/year

AADAP Contact Information:
Ms. Bonnie Johnson, FWS-AADAP
Phone: 406-994-9905
Fax: 406-582-0242
email: bonnie_johnson@fws.gov