Calcein (Se-Mark®) is a liquid that contains 1% calcein for bath marking treatments on finfish and select freshwater mussels. Calcein is only available for purchase through Syndel USA. Calcein is a fluorochrome compound that chemically binds with alkaline earth metals such as calcium, and upon binding, shows a marked increase in fluorescence when excited with blue light of about 500 nm wavelength. The primary goal of field studies conducted under INAD #10-987 is to establish the effectiveness of calcein to mark fin rays, scales, otoliths, and other calcified fish, oysters, or selected mussel tissues via immersion baths. This is a non-lethal marking evaluation method. The treatment options available for treating fish are 1) 125 - 250 milligram (mg) calcein per liter for 1 – 6 hours; or 2) 2.5 - 5.0 gram (g) calcein per liter (finfish only) for 1 - 7 minutes. Note: Treatment 2 may include a pre-treatment with a 1 - 5% salt solution for ~3.5 minutes. The treatment regimen for oyster spat is 250 mg/L for 24 hrs. Due to the treated fish and oyster size restrictions (fish <2 g and oyster spat <25mm) and limited mussel species authorized for treatment, there is no Investigational withdrawal period associated with use of calcein under this INAD. To participate in the INAD Program for calcein visit the data collecting site.
Please read the Study Protocol prior to any treatments. It contains the protocol, MSDS, and a copy of the forms to be used as a guide for collecting data that will be entered into the online INAD database. The FDA authorization letter(s) for this INAD is currently only available by emailing Bonnie Johnson .
INAD objective/purpose: Collect supportive and/or pivotal data needed to establish the effectiveness of calcein to mark fin rays, scales, otoliths, and other calcified structures in fish, oysters, or selected mussel tissues via immersion bath.
1441 W Smith Rd,
Ferndale, WA 98248 USA
Target pathogen(s): Not Applicable
Method of administration: Immersion: standing-bath treatment only
Investigational Withdrawal period:
Required test parameters:
Limitations or restrictions on use of drug:
Required INAD fee: $700/facility/year
AADAP Contact Information:
Ms. Bonnie Johnson, FWS-AADAP