Luteinizing Hormone – Releasing Hormone Analogue
Method of Administration
Required INAD Fee
Drug History and Summary
The use of hormones to induce spawning in fish is critical to the success of many federal, state, private, and tribal fisheries programs. A wide variety of programs, including many that involve the restoration of threatened/endangered species, are dependent upon hormone treatment to complete final gamete maturation and ensure successful spawning.
The time of spawning is by its own nature a stressful period for all fish species. Both sexes are undergoing significant changes in physiology, morphology, and behavior. The additional handling of fish required during the spawning process complicates an already delicate situation. This is particularly true for wildstock species that must endure the added stresses of capture, handling, and confinement in an un-natural environment. In fact, with respect to some wildstock species, the stress of capture alone is often sufficient to cause complete reproductive failure unless spawning is induced by hormone treatment. Hormone treatment in a variety of fish species is essential to facilitate optimal spawning success.
Studies have shown that final gamete maturation (ovulation and spermiation) in fish can be induced by the administration of a variety of hormones (Donaldson and Hunter 1983; Goetz 1983). Recent investigations have found luteinizing hormone-releasing hormone analogue (LHRHa) to be one of the most effective means of inducing final gamete maturation. This compound is a synthetic gonadotropin releasing hormone that is similar in structure to native luteinizing hormone-releasing hormones. LHRHa is an favored choice as a spawning aid because it has both a high biological activity and low species specificity, making it appropriate for use on a variety of fish species (Coy et al. 1974).
The primary goal of field studies conducted under INAD #8061 is to generate data to help determine appropriate LHRHa treatment regimens for inducing gamete maturation in a variety of cultured and wildstock fish species. Under this INAD, Western Chemical, Inc., is the sole authorized LHRHa supplier to all Investigators. Fish may be treated at a dose range of 5 to 100 microgram (µg) LHRHa per kilogram (kg) body weight with single or multiple IP or IM injections. Multiple injections will generally consist of a single “priming dose,” followed by a single “resolving dose.” Adherence to a 14-d withdrawal period is required before treated fish can be released or slaughtered as food fish.
Please see the LHRHa study protocol for all cited references.
Collect supportive and pivotal data needed to establish the effectiveness of LHRHa to induce gamete maturation in a variety of fish species.
Required Test Parameters
Investigator must collect data reporting percent ovulation and/or percent spermiation in treated fish. Investigator should also report general fish behavior and any adverse effects relating to treatment.
Restrictions on Use
Investigator must follow all instructions in the Study Protocol for INAD 8061 regarding drug acquisition and handling, fish treatment and disposition, and data reporting requirements.
5 - 100 microgram (µg) LHRHa per kilogram (kg) body weight
Single or multiple treatment. Multiple treatment will generally consist of a single "priming dose," followed by a single "resolving dose." Administered IP or IM.
14 days for all fish; no withdrawal period is required for injected fish that will not be susceptible to legal harvest for at least 14 days post-treatment.
Data Collection and Reporting
The following drug forms are intended primarily to provide Investigators a guide as to the type of data that must be collected during a study. In some cases, Investigators may deem the forms appropriate for the collection of raw data. However, it is extremely important to note that all data must be entered (and submitted) using the INAD Program Management System (online database). Drug Forms
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