This publication was originally produced in 2000 for the U.S. Fish and Wildlife Service, to describe the protocols and procedures utilized in the National Wild Fish Health Survey (NWFHS). The Fifth edition was completed in May of 2009 to revise chapters 5, 7, 10, 11, 12 and 15 to reflect changes in content and remove redundancy, revise changes to primer sets. New methods for KHV and changes to the revision process were added.

The NWFHS Laboratory Procedures Manual is intended for use by USFWS Fish Health Centers, but may also be helpful to fishery professionals, the technically oriented fishery industry, and students of biological or fishery sciences.

All recipients are requested to provide feedback on their use of this Manual and offer suggestions for improvements. The material may be updated annually if significant changes are made in the detection or corroborative testing methods, or in the overall Survey procedures.

To cite this publication:

Heil, N. (Ed) 2009. National Wild Fish Health Survey - Laboratory Procedures Manual. 5.0 Edition. U.S. Fish and Wildlife Service, Warm Springs, GA