Footnotes:
1. See Handbook for laboratory
methods. Actual sample size depends on population size from which samples
are being collected, the desired prevalence of infection (POI) to be detected
within that population and the desired statistical confidence interval
(CI) of the findings. An approximation of such sample schemes are provided
in 713 FW 2.2C(3).
A table (found in the Handbook), which supplements
the above table and 713 FW 2.2C.,
was extracted from Simon & Schill (1984). This supplemental table
presents guidance for more precise minimum sample sizes to be collected
for a given population size, POI and CI.
2. N/A = Not applicable
3. Sample most susceptible species
on a given water source for entire facility inspection.
4. Special cases include, but
are not limited to: warm and cool water species, threatened and endangered
species, species of concern, native fish species, animals for which there
are no commonly accepted diagnostic techniques, and low captive population
sizes (< 100 animals). Objectives for sampling program would be
based on a risk assessment and coordinated with involved Service personnel
and cooperators.
5. To achieve the necessary sample
size relative to the desired POI, the composite sample may comprise any
combination of ovarian fluid and kidney/spleen (e.g., ovarian fluid samples
from 120 fish plus individual kidney/spleen samples from 30 different fish).
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