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Exhibit 1, 713 FW 2
Aquatic Animal Inspection Sampling Schemes

FWM#: 433  (New)

Date: November 25, 2003

Series:  Fisheries

Part 713:  Fish Health

Originating Office:   Division of  the National Fish Hatchery System

  PDF Version




AQUATIC ANIMAL INSPECTION SAMPLING SCHEMES (MINIMUM REQUIREMENTS) AND REQUIRED TISSUE TYPE FOR ESTIMATING PATHOGEN PREVALENCE1

Population

Minimum Acceptable Prevalence of Infection and Tissue Type

Action Involving Population

Viruses

Renibacterium salmoninarum
(salmonids only)

Other Cultured Bacteria

Myxobolus cerebralis
(salmonids only)

captive or feral broodstock

disinfected egg transfers

2% POI

ovarian fluid and/or kidney/spleen5

5% POI

ovarian fluid and/or kidney/spleen5

optional

N/A2

primary broodstock

disinfected egg transfers

2% POI

ovarian fluid and/or kidney/spleen5

5% POI

ovarian fluid and/or kidney/spleen5

10% POI

kidney

N/A

live fish

fish transfer

5% POI

kidney/spleen

5% POI

kidney/spleen

10% POI

kidney

5% POI

gill & cranium3

special cases4

sample number, type & methods at discretion of Fish Health Center Biologist in charge


Footnotes:

1. See Handbook for laboratory methods. Actual sample size depends on population size from which samples are being collected, the desired prevalence of infection (POI) to be detected within that population and the desired statistical confidence interval (CI) of the findings. An approximation of such sample schemes are provided in 713 FW 2.2C(3). A table (found in the Handbook), which supplements the above table and 713 FW 2.2C., was extracted from Simon & Schill (1984). This supplemental table presents guidance for more precise minimum sample sizes to be collected for a given population size, POI and CI.

2. N/A = Not applicable

3. Sample most susceptible species on a given water source for entire facility inspection.

4. Special cases include, but are not limited to: warm and cool water species, threatened and endangered species, species of concern, native fish species, animals for which there are no commonly accepted diagnostic techniques, and low captive population sizes (< 100 animals). Objectives for sampling program would be based on a risk assessment and coordinated with involved Service personnel and cooperators.

5. To achieve the necessary sample size relative to the desired POI, the composite sample may comprise any combination of ovarian fluid and kidney/spleen (e.g., ovarian fluid samples from 120 fish plus individual kidney/spleen samples from 30 different fish).


For information on the specific conent of this exhibit, contact the Division of the National Fish Hatchery System. For questions about this Web page, contact Krista Bibb , in the Division of Policy and Directives Management.  


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