Fact Sheet: Calcein INAD 10-987

for detailed information, see INAD study protocol, use link on the left
last updated: 1 December 2011

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Authorization & Categorical Exclusion Letters

Objective/purpose: Collect supportive and pivotal data needed to establish the effectiveness of calcein to mark fin rays, scales, otoliths, and other calcified fish or selected mussel tissues, via immersion baths.
Drug name: SE-MARK® (Calcein)
Source of drug: Western Chemical, Inc.


1269 Lattimore Road
Ferndale, WA 98248 USA


Attention: Jason Montgomery
Toll Free: 800-283-5292 ext 4175
email: jasonm@wchemical.com
webpage: http://www.wchemical.com/

Target pathogen(s): Not Applicable
Method of administration: Immersion: standing-bath treatment only
Treatment dosage: Option A: 125 - 250 milligrams calcein per liter
Option B: 2.5 - 5.0 grams calcein per liter (finfish only)
Treatment regimen:

Option A: Treatment duration is 1 - 6 hours
Option B: Treatment duration is 1 - 7 minutes (Note: Treatment may include a pre-treatment with a 1 - 5% salt solution for ~3.5 minutes.)

Calcein may be applied as a single treatment, or repeated treatments.

Withdrawal period: None for fish; they may be released immediately following treatment for those treated at less than 2 grams and for Federally Threatened and Endangered species.

None for mussels; due to their treatment at an early life stage and the limited human consumption
Required test parameters: Investigator must collect mark retention and mortality data. Investigator should also report general fish behavior and any adverse effects relating to treatment.
Limitations or restrictions on use:

Treatment is restricted to finfish having a body weight of 2 grams or less.

Treatment of mussels is restricted to the following species: Higgins eye, hickory nut, black sandshell, pocketbook, fat mucket, sheepnose and maple leaf.

Repeated treatments may be conducted to establish mutltiple marks. However, an interval of at least 2 days should be observed between treatment events.

No discharge of calcein marking solution is allowed. Although used calcein marking solution may be stored on station in a secure, leak-proof container, it must ultimately be disposed of according to procedures detailed in a general Waste-stream profile (see INAD Study Protocol for specific instructions).

Investigator must follow all instructions in the Study Protocol for INAD 10-987 regarding drug acquisition and handling, fish treatment and disposition, and data reporting requirements.

Required INAD fee: $400.00 per facility per year
AADAP contact for other information: Ms. Bonnie Johnson, FWS - AADAP
Phone: 406-994-9905
Fax: 406-582-0242

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