Red-cockaded woodpecker - Picoides borealis (E)(h)
 Mountain blackside dace - Phoxinus cumberlandensis (T)
 Duskytail darter - Etheostoma (Catonotus) percnurum (E)
 Little-winged pearly mussel - Pegias fabula (E)
 Cumberland bean pearly mussel - Villosa trabalis (E)
 Cumberland elktoe - Alasmidonta atropurpurea (E)(CH)
 Cumberland combshell - Epioblasma brevidens (E)(CH)
 Oyster mussel - Epioblasma capsaeformis (E)(CH)
 Cumberland sandwort - Arenaria cumberlandensis (E)
 Virginia spiraea - Spiraea virginiana (T)
 Cumberland rosemary - Conradina verticillata (T)
 Cumberland Johnny darter - Etheostoma nigrum susanae (C)
 Tan riffleshell - Epioblasma walkeri (E)