Gray bat - Myotis grisescens (E)
 Red-cockaded woodpecker - Picoides borealis (E)(h)
 Snail darter - Percina tanasi (T)
 Yellowfin madtom - Noturus flavipinnis (T)(h)
 Dromedary pearly mussel - Dromus dromas (E)(h)
 Pink mucket pearly mussel - Lampsilis abrupta ( =Lampsilis orbiculata)(E)
 Orangefoot pimpleback - Plethobasus cooperianus (E)(h)
 Birdwing pearly mussel - Conradilla caelata (=Lemiox rimosus) (E)(h)
 Fine-rayed pigtoe - Fusconaia cuneolus (E)(h)
 Cumberland bean pearly mussel - Villosa trabalis (E)(h)
 Tan riffle shell - Epioblasma walkeri (E)(h)
 Tuberculed-blossom pearly mussel - Epioblasma torulosa torulosa (E)(h)
 Green-blossom pearly mussel - Epioblasma torulosa gubernaculum (E)(h)
 Turgid-blossom pearly mussel - Epioblasma turgidula (E)(h)
 Shiny pigtoe - Fusconaia edgariana (=Fusconaia cor) (E)(h)
 Rough pigtoe - Pleurobema plenum (E)(h)
 Cumberland monkeyface pearly mussel - Quadrula intermedia (E)(h)
 Yellow-blossom pearly mussel - Epioblasma florentina florentina (E)(h)
 Cumberland rosemary - Conradina verticillata (T)(h)
 Anthony's river snail - Athearnia anthonyi (E)(h)
 Sheepnose -  Plethobasus cyphyus (C)