Gray bat - Myotis grisescens (E)
 Slender chub - Erimystax cahni (T)(CH)
 Yellowfin madtom - Noturus flavipinnis (T)(CH)
 Pygmy madtom - Noturus stanauli (E)
 Birdwing pearly mussel - Conradilla caelata (=Lemiox rimosus) (E)
 Dromedary pearly mussel - Dromus dromas (E)
 Green-blossom pearly mussel - Epioblasma torulosa gubernaculum (E)(h)
 Shiny pigtoe - Fusconaia edgariana (=Fusconaia cor) (E)
 Fine-rayed pigtoe pearly mussel - Fusconaia cuneolus (E)
 Cumberland monkeyface pearly mussel - Quadrula intermedia (E)
 Appalachian monkeyface pearly mussel - Quadrula sparsa (E)
 Rough pigtoe - Pleurobema plenum (E)
 Pink mucket pearly mussel - Lampsilis orbiculata (E)
 Yellow-blossom pearly mussel - Epioblasma florentina florentina (E)(h)
 Cracking pearly mussel - Hemistena lata (E)
 Fanshell - Cyprogenia stegaria (E)
 Cumberlandian combshell - Epioblasma brevidens (E)(CH)
 Oyster mussel - Epioblasma capsaeformis (E)(CH)
 Rough rabbitsfoot - Quadrula cylindrica strigillata (E) (CH)
 Slabside pearly mussel - Lexingtonia dolabelloides (C)(CH)
 Fluted kidneyshell - Ptychobranchus subtentum (C)(CH)
 Rough rabbits foot - Quadrula cylindrica strigillata (E)(CH)
 Purple bean - Villosa perpurpurea (E)(CH)
 Spectaclecase - Cumberlandia monodonta (C)
 Sheepnose -  Plethobasus cyphyus (C)