Indiana bat - Myotis sodalis (E)
 Red-cockaded woodpecker - Picoides borealis (E)(h)
 Spotfin chub - Erimonax monachus (T)(CH)
 Cumberland elktoe - Alasmidonta atropurpurea (E)
 American chaff-seed - Schwalbea americana (E)(h)
 Cumberland sandwort - Arenaria cumberlandensis (E)
 Cumberland rosemary - Conradina verticillata (T)
 White fringeless orchid - Platanthera integrilabia (C)
 Virginia spirea - Spiraea virginiana (T)
  Green pitcher plant - Sarracenia oreophila (E)(h)
  Gray bat - Myotis grisescens (E)