California-Nevada Fish Health Center
California and Nevada Organization

National Wild Fish Health Survey - Laboratory Procedures Manual

Second Edition- June 2004

PLEASE READ THIS ENTIRE SECTION PRIOR TO USING OR PRINTING THIS MANUAL

APPROPRIATE USE OF THIS PUBLICATION

This publication was originally produced in 2001, the Second Edition in 2004, for the U.S. Fish and Wildlife Service, to describe the protocols and procedures utilized in the National Wild Fish Health Survey (NWFHS). It is intended for use by Fish Health Centers, but may also be helpful to fishery professionals, the technically oriented fishery industry, and students of biological or fishery sciences.

Distribution will be to select libraries and fishery-related agencies in the United States. Copies may be requested from the U.S. Fish and Wildlife Service by writing to:

Kimberly True
U.S. Fish and Wildlife Service
California-Nevada Fish Health Center
24411 Coleman Hatchery Road
Anderson, CA 96007

All recipients are asked to provide feedback on their use of this Manual and offer suggestions for improvements. This material may be updated in the future if significant changes are made in the Survey procedures.

Because sections in this publication may not have undergone full editorial processing and complete peer review and may be published elsewhere in the formal literature, they should not be cited without approval of the author.

NOTE: The individual chapters comprising this Manual are to be used only within the context of the entire Manual. While chapters are presented as individual PDF files, they are not intended to be used separately. It is recommended to print all chapters and assemble the Manual in a loose-leaf binder.

Please read Chapter 1 - Overview of the National Wild Fish Health Survey in it's entirety prior to using this Manual or the procedures listed within. Chapter 1 explains the intent and guiding principles of the Survey including: target pathogens, diagnostic and corroborative protocols, and data management in the NWFHS Database.

Preface

Instructions for Using & Printing the Manual

 

Table Of Contents, Please contact us at redbluff@fws.gov if you need this document in another format
Sample Receipt and Laboratory Tracking, Please contact us at redbluff@fws.gov if you need this document in another format
Enzyme Linked Immunosorbent Assay (ELISA) for Detection of Renibacterium salmoninarum, Please contact us at redbluff@fws.gov if you need this document in another format
Corroborative Testing of Parasites by Polymerase Chain Reaction (PCR), Please contact us at redbluff@fws.gov if you need this document in another format
Corroborative Testing of Viral Isolates, Please contact us at redbluff@fws.gov if you need this document in another format
Manual Revision Procedures, Please contact us at redbluff@fws.gov if you need this document in another format
Overview of the National Wild Fish Health Survey, Please contact us at redbluff@fws.gov if you need this document in another format
Standard Necropsy Procedures for Finfish, Please contact us at redbluff@fws.gov if you need this document in another format
Please contact us at redbluff@fws.gov if you need this document in another format
Tissue Culture of Fish Cell Lines, Please contact us at redbluff@fws.gov if you need this document in another format
Histology for Finfish, Please contact us at redbluff@fws.gov if you need this document in another format
Sample Collection & Submission, Please contact us at redbluff@fws.gov if you need this document in another format
Bacteriology, Please contact us at redbluff@fws.gov if you need this document in another format
Parasitology, Please contact us at redbluff@fws.gov if you need this document in another format
Virology, Please contact us at redbluff@fws.gov if you need this document in another format
Non-Lethal Methods for Detection of Fish Pathogens, Please contact us at redbluff@fws.gov if you need this document in another format
Last updated: September 23, 2008