Welcome to Quality Improvement Studies
The Fish and Wildlife Service Aquatic Animal Health Program (FWS-AAHP), in
partnership with the American Fisheries Society Fish Health Section (AFS-FHS)
revisions of the Standard Procedures for Aquatic Animal Health Inspections
to ensure methodologies are current and effective in determining the disease
of fish populations.
Similarly, the FWS Fish Health Center specialists review and update the National
Wild Fish Health Survey Manual annually to incorporate new techniques with improved
sensitivity for detecting fish pathogens in wild fish populations.
The FWS-AAHP is committed to excellence in science, and often conducts Quality
Improvement Studies to ensure current fish pathogen detection methods, and
newly developing techniques, meet high standards. Often new literature and
technologies suggest changes in sampling procedures, reagents, or assay protocols
will provide improved detection of fish pathogens. Fish health specialists
conduct comparative studies to ensure that proposed changes to current methodologies
will produce accurate and consistent laboratory test results. Many of the
FWS Fish Health Centers also conduct applied research to address specific
health issues at local hatcheries, or regional information needs for restoration
Completed Quality Improvement Studies
Several studies have been completed in the past few years to address disease
sampling techniques, improve reagent reference material, and to validate new
technologies such as quantitative PCR. Two studies are currently underway to
improve sampling and testing efficiency while maintaining sensitivity.
||Pooling Study – Aeromonas salmonicida detection methods
in naturally infected Lahontan cutthroat trout. (CaNvFHC and Lahontan
||Pooling Study – Yersinia ruckeri in artificially infected
rainbow trout. (OFHC, USGS, and North Carolina State College)
||Comparative Study to validate the sensitivity of Quantitative PCR for
detection of Renibacterium salmoninarum compared to the ELISA assay.
(CaNvFHC). – Report in progress
||Field Testing of PCR Positive Control (quantified DNA) for use in Renibacterium
salmoninarum Quantitative PCR. (CaNvFHC, ATCC).
In Progress and Planned Studies for 2005
||Pooling Study – Renibacterium salmoninarum to compare sensitivity
of two assays in relationship to pool size. (CaNvFHC – in progress)
||Evaluation of PCR sensitivity comparing cranial tissue and spore digests.
(Bozeman FHC – in progress)
||DNA Extraction Efficiency (Abernathy Fish Technology Center (AFTC) and
||Spiked Kidney Tissue with ATCC Rs+ PCR Control (CaNv FHC and OFHC)